![]() ![]() Virus Res 175(1):30–44īlacklaws BA, Berriatua E, Torsteinsdottir S, Watt NJ, de Andres D, Klein D, Harkiss GD (2004) Transmission of small ruminant lentiviruses. Santry LA, De Jong J, Gold AC, Walsh SR, Menzies PI, Wootton SK (2013) Genetic characterization of small ruminant lentiviruses circulating in naturally infected sheep and goats in Ontario, Canada. Greenwood PL, North RN, Kirkland PD (1995) Prevalence, spread and control of caprine arthritis-encephalitis virus in dairy goat herds in New South Wales. Proc Natl Acad Sci USA 82(20):7086–7090ĭe Boer GF, Terpstra C, Houwers DJ, Hendricks J (1979) Studies in epidemiology of maedi/visna in sheep. Gendelman HE, Narayan O, Molineaux S, Clements JE, Ghotbi Z (1985) Slow, persistent replication of lentiviruses: role of tissue macrophages and macrophage precursors in bone marrow. This study is the first report to describe the molecular characteristics of CAEV circulating in the Philippines. Molecular characterization of the CAEV gag gene is important for the development of a detection kit specific for the local strain of CAEV and the establishment of small ruminant lentivirus eradication programs in the Philippines. Based on amino acid sequence alignments, it is possible that the Philippine CAEV isolate is a new strain of CAEV, but it is also possible that it was already present in the country even before the start of goat importation. ![]() In addition, the Philippine CAEV isolate clustered in group B with the prototype caprine lentivirus. CAEV was detected using nested polymerase chain reaction (PCR), and new sets of primers were designed in order to amplify the gag gene, which is a highly conserved region of the viral genome. DNA sequence analysis showed homology of 86–93 % between Philippine CAEV and available CAEV sequences in GenBank. Caprine arthritis encephalitis virus (CAEV) causes caprine arthritis encephalitis syndrome, which is an emerging disease of goats in the Philippines. ![]()
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